The expression of these cDNAs in the transfectants was shown at transcriptional levels by RT-PCR, e.g., EBOLA GP [92] and, in most cases, at protein levels by Western blot analysis, e.g., HIV GP120, SARS-CoV-2 spike and membrane proteins, melanocytic PRAME, CAGE cancer antigens, and enolase 1 (ENO1) of lung and pancreatic cancers. The gene discussed is DDX53; the disease is familial pancreatic carcinoma.