With this purpose, Berthuy et al. fabricated a biosensing system composed of an Au-slide provided with specific Abs spots for the real-time identification of prostate-specific antigen (PSA), involved in the phases of prostate cancer, and β2-microglobulin (β2M) released by a human prostate carcinoma cell line (LNCaP) embedded in 3D alginate hydrogels under dihydrotestosterone (DHT) stimulation. Here, KLK3 is linked to prostate cancer.