Prostate cancer cells were cultured with CM from HS5 bone marrow stromal cells and treated with the antioxidant N-acetylcysteine (NAC), resulting in a loss of the elevated G6PD expression in response to bone marrow stromal cells and suggesting that the increase in ROS could be further driving the elevated G6PD (Fig. 5I). This evidence concerns the gene G6PD and Familial prostate cancer.