We found that the percentages of GDCA in HCC patients were significantly decreased, the percentages of TUDCA, TDCA and THDCA were all significantly decreased in DEN-induced HCC mice, and the percentages of UDCA, TDCA, and THDCA were significantly decreased in vancomycin-treated mice with HCC. This evidence concerns the gene GNAT3 and hepatocellular carcinoma.