Finally, to further confirm the interaction between TYMSOS and miRNA-214-3p, the luciferase assay was performed, the luciferase assay indicated that transfection of miRNA-214-3p mimics significantly decreased the relative luciferase activity of TYMSOS-WT–treated lung cancer cells, but did not affect that of TYMSOS-MUT–treated lung cancer cells (Figure 11G). The gene discussed is TYMSOS; the disease is lung cancer.