Figure 5(b) shows that between amino acids 0 and 183, a total of 29 MFAP2 mutation sites, including 27 missense and 2 splices, were detected, with missense being the predominant type of DNA alteration. Of these, F157 L in the ShKr protein domain was the most frequent mutant site, detected in 2 cases of endometrial carcinoma. Moreover, the MFAP2 mutant sites were further demonstrated in the 3D structure as shown in Figure 5(c). Additionally, we found that MFAP2 expression was independent of mutations (Figure 5(d)) and independent of DNA copy variation Figure (5(e)). The gene discussed is MFAP2; the disease is endometrial carcinoma.