The LS is thought to regulate anxiety through its outputs to the hypothalamus, and a recent study showed that a subset of GABAergic LS neurons expressing type 2 corticotropin-releasing factor receptor (Crfr2) project to the anterior hypothalamic area (AHA) of the medial hypothalamus, and that optogenetic stimulation of the LSCrfr2+-AHA circuit promotes anxiety-related behaviors and increases corticosterone levels [69]. This evidence concerns the gene CRHR2 and Anxiety.