We detected the expression levels of these 14 mRNAs in miR-527-knockdown HCC cells, and among the 14 upregulated genes identified in this manner, only ubiquitin-conjugating enzyme E2A (UBE2A) expression was most prominently upregulated (Fig. 5B), suggesting that UBE2A expression may be most likely affected by miR-527. The gene discussed is UBE2A; the disease is hepatocellular carcinoma.