Although upon infection there has been a drop in expression of HRS or Rab27a proteins that are known to have role in late endosome/MVB formation and EV release (Fig 4A and B), we did not detect a significant change in EV diameter or marker protein levels in population isolated from both control and Ld-infected RAW264.7 cells (Fig 4C–E). The gene discussed is RAB27A; the disease is infection.