AKT1 and cancer: In this in vivo experiment, we found that the combination of Rhein and RAD001 significantly reduced tumor weight and volume, repressed PI3K/AKT/mTOR pathway activation, and decreased tumor cell proliferation marker Ki-67 expression than the administration of Rhein or RAD001 alone, which indicated RAD001 and Rhein combination exerted synergistic cancer prevention on GC in vivo xenograft model.