To test the ability of FLIm to detect inflammation we used dextran sodium sulfate (DSS) to induce colitis on the mouse colon and 5-aminosalicylic acid (5-ASA), a PPAR-γ (peroxisome proliferator-activated receptor gamma) agonist that acts on the colon epithelial cells, to mitigate the inflammatory effects. Here, PPARG is linked to colitis.