In a previous study, the mechanisms of DMC-induced cell apoptosis, cell death, and G0/G1 cell cycle arrest were found to involve the PI3K/AKT mitochondria-dependent pathway and increased generation of reactive oxygen species (ROS) in hepatocellular carcinoma (BEL-7402 and BEL-7402/5-FU). The gene discussed is AKT1; the disease is hepatocellular carcinoma.