Next, we examined the potential association of NRF2 and its related genes, the CSC markers EpCAM and CD133 with following clinicopathological variables of HCC: gender, age, smoking, alcohol consumption, serum AFP level, tumor size, ES grade, number of tumors, vascular invasion, pathology stage, cirrhosis, viral status, and metastasis. The gene discussed is AFP; the disease is hepatocellular carcinoma.