The examples of negative and positive expressions of IL4Rα and IL13Rα1 in adenocarcinoma and squamous cell carcinoma components of gallbladder carcinoma are shown in Figure 1A. The cut-off points for the immunohistochemical staining scores for the nuclear expression of IL4Rα (Nu-IL4Rα), cytoplasmic expression of IL4Rα (Cy-IL4Rα), nuclear expression of IL13Rα1 (Nu-IL13Rα1), and cytoplasmic expression of IL13Rα1 (Cy-IL13Rα1) were determined by ROC curve analysis (Figure 1B). This evidence concerns the gene IL13RA1 and squamous cell carcinoma.