The results of this study suggest that PPARG (rs1801282), TMEM163 (rs6723108 and rs998451), UBE2E2 (rs6780569), and WFS1 (rs4689388) gene polymorphisms are not significant risk factors for GDM development in the Polish population and do not affect the clinical parameters in women with GDM, only rs1801282 of PPARG gene may influence BMI values in women with GDM. Here, TMEM163 is linked to gestational diabetes.