The validation cohort differed significantly from the derivation cohort in terms of age, HF duration and symptoms, comorbidities (obesity—body mass index, dyslipidaemia, anaemia and chronic kidney disease), heart rate, NT-proBNP and their required loop diuretics dosage (Table S1, Appendix B). The gene discussed is NPPB; the disease is obesity due to melanocortin 4 receptor deficiency.