In order to address the biological functions of C1GALT1 in CCA, endogenous C1GALT1 expression was examined in four CCA lines (KKU-055, KKU-100, KKU-213A, and KKU-213B) and an immortalized cholangiocyte (MMNK-1) using western blot analysis. This evidence concerns the gene C1GALT1 and cholangiocarcinoma.