Subsequently, the following parameters were evaluated: TMZ resistance (as evaluated by MGMT pyrosequencing); cancer stem cell marker (CD133 and SOX2); half-maximal inhibitory concentrations (IC50) of BP, BCNU, and MTIC (the active form of TMZ); PD-L1 expression levels; and immune responses (co-culture with immune cells). This evidence concerns the gene PROM1 and cancer.