CASP3 and colorectal carcinoma: To understand the kinetics of apoptosis induction mediated by perhexiline on CRC cell lines, we treated HT29 cells grown as a monolayer with various concentrations of (−)-P, (+)-P and (R)-P and performed live-cell imaging to continuously monitor cell growth for 72 h in the presence of Caspase-3/7 Green reagent.