In 3D culture of BC cells HMT-3522, organized tissue morphology has been altered with NFKB- mediated transcription activation of the disorganization related genes that have potential NFKB binding sites in their promoter region [83], and upregulation of disorganization related genes are positively correlated with an invasive and aggressive phenotype in 3D cultures of multiple BC cell lines. This evidence concerns the gene NFKB1 and breast cancer.