Figure 4D shows a comparison of ROC curves of the derived gene panels and serum AFP. The combinations of each of the five genes and serum AFP showed no significant differences in AUROC compared to serum AFP alone (Figure 4D; middle). On the other hand, for the combination of the five genes, HMGA1 combined with MPLZ1 produced the most accurate results for predicting HCC recurrence (AUC = 0.807, 95% CI = 0.681–0.899) (Figure 4D; right). The AUC value, 95% CI, and p value of each factor are listed in Supplementary Tables S5 and S6. The gene discussed is AFP; the disease is hepatocellular carcinoma.