Genomes sequences of each isolate were screened for mutations in genes conferring resistance to first-line anti-tuberculosis drugs viz; rpsL, rrs, gidB for STR; KatG, inhA, ahpA, fab, ndh for INH; rpoA, rpoB, rpoC for RIF; embABC for EMB and pncA for PZA. The gene discussed is GLIS3; the disease is tuberculosis.