All 65 sequenced genomes were also analyzed for mutations in genes conferring resistance to second-line anti-tuberculosis drugs viz gyrA, gyrB for FQ; rrl and rplC for linezolid; Rv0678, Rv2535c, Rv1979c and mmpl5 for clofazimine; alr, ddl, ald and cycA for cycloserine; rrs for AMK; Rv0678 and atpE for bedaquiline; thyA, ribD and folC for PAS; fgd, ddn, fbiA, fbiB and fbiC for delamanid. The gene discussed is ATP5F1E; the disease is tuberculosis.