Considering that glial cells have been shown to contribute to ALS motor neuron toxicity and NF pathology [23,24,25,26,27,28,29,30], (Figure 1E), we next tested whether the presence of astrocyte and/or microglia-conditioned medium (ACM and MCM, respectively) could impact NF expression or accumulation. Here, NFASC is linked to amyotrophic lateral sclerosis.