Since de novo gene variants have been considered a major cause of early-onset genetic disorders such as ASD [19], Pathway Studio software v12.3 (https://mammal.pathwaystudio.com/, accessed on 24 September 2020) was used to identify the cell processes related to the encoded protein of the novel de novo mutation in the MIS18BP1 gene detected in our study. Here, MIS18BP1 is linked to hereditary disease.