From the barrier function assessment, the overexpression of HAVcR-1 in non-aggressive prostate cancer cells led to little difference in the physical barrier function (TER/Rb/alpha), but there was a significant increase in the paracellular permeability, indicating a reduction in a robust extracellular route, allowing the passage of substances of all sizes. The gene discussed is HAVCR1; the disease is prostate carcinoma.