Interestingly FZD8 is upregulated in advanced prostate cancer compared to benign prostate tissue, and FZD8 knockdown in DU-145 and PC3-M cells significantly reduces cell migration and invasion, associated with downregulation of mesenchymal markers seen in both in vitro cell line studies and cell line-derived organoids [99], suggesting FZD8 mediates prostate cancer dissemination and epithelial-to-mesenchymal transition (EMT). This evidence concerns the gene FZD8 and Familial prostate cancer.