Pirola et al. compared liver biopsies from NAFLD patients with healthy individuals and found that the mitochondria-encoded NADH dehydrogenase 6 (MT-ND6) has been shown to be significantly hypermethylated in patients with NASH in comparison with those who had simple steatosis, followed by decreased mRNA and protein MT-ND6 levels [244]. This evidence concerns the gene MT-ND6 and metabolic dysfunction-associated steatohepatitis.