True prevalence values estimated for infections detected by Dengue IgM were 0.134 (95% CI: 0.08–0.2), Chikungunya 0.084 (95% CI: 0.05–0.13), Scrub 0.043 (95% CI: 0.01–0.09), Japanese Encephalitis 0.045 (95% CI: 0.02–0.09). Here, CD40LG is linked to Japanese encephalitis.