Moreover, methylanthraquinones from H. diffusa induced cell apoptosis by changing Fas and Fas Ligand (Fas/FasL) and activating caspase−8 in THP−1 cells of human leukemia [18] or increased Ca2+ concentration in human breast cancer cells through phosphorylation of c-Jun N-terminal kinase (JNK) protein kinase and activation of calpain to promote cell apoptosis [19]. The gene discussed is FAS; the disease is breast cancer.