In this study, we found 12 transcription factors (STAT5A, STAT6, RUNX1, REL, SMAD3, CEBPB, CEBPD, GABPB2, FOXO1, PAX6, FOXJ1 and NOG) that are extremely important for the pathogenesis of ALS and four key prognostic markers (TPST1, IFNGR2, MAEA, ALAS2) (Figure 12). This evidence concerns the gene ALAS2 and amyotrophic lateral sclerosis.