TNF and amyotrophic lateral sclerosis: To investigate the relationship between the DEGs and the occurrence and development of ALS, we performed KEGG analysis and found that the up-regulated genes in the nerve tissue of ALS patients were mainly related to immunity and inflammation (such as cell adhesion molecules, hematopoietic cell lineage, Th17 cell differentiation, phagosome, Th1 and Th2 cell differentiation, NF-κB signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, and antigen processing and presentation).