Next, we drew heatmaps and boxplots for the four risk survival-related markers to analyze their expression levels and found that MAEA, TPST1, and IFNGR2 were highly expressed in high-risk ALS patients, while ALAS2 was highly expressed in low-risk ALS patients (Figure 11C,D). This evidence concerns the gene TPST1 and amyotrophic lateral sclerosis.