For example, PKC γ or TLR4 genes knockdown or microinjection of related inhibitors into the PVN should be performed in vitro and/or in vivo, which can directly prove that NKA α2 regulates Rac1-dependent oxidative stress and TLR4-induced inflammation in the PVN during the development of high salt-induced hypertension. The gene discussed is TLR4; the disease is Hypertension.