Another study investigated the effect on the NRF2 pathway of HFD-induced (39.7 kcal% fat) obesity among Nfe2l2-KO, WT, and Keap1-KD mice and concluded that the genetic alteration of Nfe2l2 does not prevent diet-induced obesity in mice [73]. The gene discussed is NFE2L2; the disease is obesity due to melanocortin 4 receptor deficiency.