GPT and metabolic dysfunction-associated steatotic liver disease: Obese mice presented with elevated Alanine Transaminase (ALT) levels in their plasma (Figure 1B) and decreased AST/ALT (AST: Aspartate Transaminase) ratios (Figure 1C), indicating liver damage as well as lipid deposition in the liver (Figure 1D), both reminiscent of NAFLD.