One group has found, in a mouse model of GBM, that responders to anti-PD-1 treatment combined with radiation increased the CD8+ T-cell-to-Treg ratio [146], and another group demonstrated that blocking CTLA-4 increased CD4+ T-cell proliferative capacity and decreased Treg-mediated immune suppression [172]. The gene discussed is CTLA4; the disease is glioblastoma.