Additionally, the relatively long duration of human cancer compared with the duration of murine models (typically measured in a few weeks) may prevent the mouse systems from emulating phenomena such “exhausted T cells”, particularly TILs, which have upregulated immune checkpoint molecules, including CTLA-4, PD-1, Indoleamine 2,3-dioxygenase (IDO1), T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), and Lymphocyte-activation gene 3 (LAG-3) [149]. This evidence concerns the gene CTLA4 and cancer.