GAL and depressive symptom measurement: We analyzed C-Fos IR 90 min after the coadministration of GAL(1–15) (0.3 nmol) and ESC (2.5 mg/kg) in several nuclei involved in depression and AUD—RMTg, LHb, mHb, NAc, PFC—and by double immunohistochemical staining of 5HT/C-Fos in DR or TH/C-Fos in VTA (Figure 5A,B).