We then, performed Western blotting to detect the presence of pathological TDP-43 by using an antibody against the C-t epitope of TDP-43 (Proteintech, 67345-1-Ig), and anti-flotillin-1 in cell lysates and EVs from control and AD lymphoblasts (Figure 7B). The gene discussed is FLOT1; the disease is Alzheimer disease.