DEFB1 and neoplasm: Moreover, according to the International Tumor Budding Consensus Conference 2016, three different budding grades are classified as follows: Bd1 (0–4 buds/0.785 mm2), Bd2 (5–9 buds/0.785 mm2), and Bd3 (10 or more buds/0.785 mm2) [61].