We found that the expression levels of CLSTN2, NMB, SNAP91, PCSK1N, INA and LHX6 were positively correlated with the overall survival of glioblastoma, while the expression levels of IGFBP2, GPRASP1, C1R, CHRM3, NELL1, SEZ6L2, ICAM5, HPCAL4, PGBD5 and UCHL1 were negatively correlated with overall survival. The gene discussed is HPCAL4; the disease is glioblastoma.