We used three approaches to define the role of NLRP7: (i) a clinical study in which we used human sera and placentae that were collected from normal pregnant women and from patients with CHM or CC; (ii) an in vitro study in which we investigated the influence of NLRP7 knockdown on the tumorigenesis of the choriocarcinoma cell line, JEG3, which used both 2D and 3D culture systems; and (iii) an in vivo study in which we used an orthotopic model of CC and a metastatic model of this cancer [65]. Here, NLRP7 is linked to cancer.