In addition, our RNA-seq analysis indicated that impaired VLDL secretion and SREBP1c mediated DNL is unlikely to be the cause of hepatic steatosis in the STAM mouse model because VLDL assembly regulated Mttp was induced, insulin and SREBP1c target gene of Gck was reduced, and Pck1, which is negatively regulated by insulin, was induced. Here, INS is linked to fatty liver disease.