In the present study, following treatment, two proteins (CPN and C3) were up-regulated, and seven proteins (i.e., albumin precursor, AGT, ACT-1, and fetuin-B) were down-regulated, showing the treatment-related changes in serum proteome patterns of dogs with CHF in parallel to improvement of their clinical conditions. This evidence concerns the gene TRAF3IP2 and congestive heart failure.