To further validate these results, we performed EdU staining in two patient‐derived GSC cell lines cultured in stem cell media (U3024MG, U3031MG), and in another GBM cell line cultured in media with 10% FBS (U2987MG), but starved in the current experiments prior to TGFβ treatment. The gene discussed is TGFB1; the disease is glioblastoma.