CD40LG and infection: Furthermore, following vaccination the SpAb responses showed strong negative trends to the final fluke burdens, notably following vaccination and on the day of infection for both IgG (r = −0.77, p = 0.07) and IgM (r = −0.75, p = 0.08) with trends not observed at the same time points in the control group, indicating a potential link between vaccine-induced anti-GST antibodies and final fluke levels.