Results of univariate analysis indicated that Gal-9 expression (P =0.002), tumor stage (P =0.003), lymph node metastasis (P <0.001), distant metastasis (P =0.040) and TNM stage (P <0.001) were closely associated with the prognosis of HBV-associated HCC patients. This evidence concerns the gene LGALS9 and neoplasm.