ASCL1 and colorectal carcinoma: Closer inspection of the network also revealed the presence of ASCL1, another member of the MES CRC TFs, which also displayed negative regulation with hsa-mir-431 (p = 3.58E−6, correlation = − 0.61), hsa-mir-432 (p = 1.7E-4, correlation = − 0.52) and hsa-mir-433 (p = 1.48E–5, correlation=− 0.58) (Additional file 5: Fig. S4C).