In summary, the MIR4435-2HG/hsa-miR-1-3p/MMP9/hsa-miR-29-3p/DUXAP8 ceRNA network axis was constructed by using bioinformatical tools, and it might be closely associated with the development of HCC, associated with a poor prognosis in HCC patients, and associated with tumor immune cell infiltration. Here, MMP9 is linked to hepatocellular carcinoma.