Combined with our previous findings that the effects of NOV on the inhibition of CRC cells proliferation was mediated by a regulation of Caspase-3/-8 via JNK [17], both in vitro and in vivo results in this study suggested that JNK/AP-1/Caspase-8/Caspase-3 was a possible signaling pathway involved in the sensitization of NOV on 5-Fu in treating CRC. The gene discussed is CASP8; the disease is colorectal carcinoma.