Analysis of TCF21 expression in different adrenocortical tumor cell cultures (Fig. 1A) showed lower mRNA expression of TCF21 in human ACC cells; H295R cells and ACC-T36 cells when compared to cell culture from pediatric adenoma, ACAPed-T7 and human normal adrenal pool (NA), which was used as reference. Here, TCF21 is linked to adrenal cortex carcinoma.