DIAPH3 and osteosarcoma: The OD450 nm values of MG-63-shDIAPH3 and HOS-shDIAPH3 cells at 48 h and 72 h were significantly lower than those of MG-63-NC and HOS-NC cells (Fig. 2C), indicating that DIAPH3 knockdown suppressed the proliferation of osteosarcoma cells.