The percentage of MG-63-shDIAPH3 and HOS-shDIAPH3 cells in the G1 phase was significantly higher than that in MG-63-NC and HOS-NC cells (Fig. 2D), indicating that DIAPH3 knockdown induced cell cycle arrest in the G1 phase of osteosarcoma cells. This evidence concerns the gene DIAPH3 and osteosarcoma.