DIAPH3 and osteosarcoma: The number of invaded MG-63-shDIAPH3 and HOS-shDIAPH3 cells in the Transwell Matrigel assay was significantly lower than that of migrated MG-63-NC or HOS-NC cells (Fig. 3C), indicating that DIAPH3 knockdown suppressed the invasion of osteosarcoma cells.