In Figure 3b after flow cytometry assay, it was found that HeLa and HepG2 cells were treated with Sdy-1 for 48 h, the number of Annexin V-positive cells increased from 15.20%, 7.82% to 42.50% and 26.00%, and Annexin V-positive cells increased significantly, suggesting that Sdy-1 promoted tumor cell apoptosis. Here, ANXA5 is linked to neoplasm.